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The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided.Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits. Crispr Cas A Laboratory Manual Download eBook pdf epub Download crispr cas a laboratory manual or read online books in PDF EPUB Tuebl and Mobi Format CRISPR-Cas: A Laboratory Manual by - EbookNetworkingnet CRISPR-Cas: A Laboratory Manual a book by CRISPR-Cas: The development of CRISPR-Cas technology is revolutionizing biology CRISPR-Cas a laboratory manual (Book 2016) [WorldCatorg] CRISPR-Cas a laboratory manual [Jennifer Doudna; Prashant Mali;] Home WorldCat Home About WorldCat Help Search Search for Library Items Search for Lists Search CRISPR-Cas A Laboratory Manual - satchgolforg CRISPR-Cas A Laboratory Manual BOOK DETAILS Author : Pages : 192 Pages Publisher : Cold Spring Harbor Laboratory Press Language : English CRISPR-Cas: A Laboratory Manual - CRISPR-Cas: A Laboratory Manual: Featuring contributions from leading figures in the CRISPR-Cas field this laboratory manual presents a state-of-the-art guide to CRISPR-Cas : a laboratory manual New Arrival Textbooks CRISPR-Cas : a laboratory manual ; Advanced organic chemistry ; TOEFL ITP test listening grammar & reading ; Epigenetic gene expression and regulation ; CRISPR-Cas: A Laboratory Manual - Hardcover - Jennifer beforeFeaturing contributions from leading figures in the CRISPR-Cas field this laboratory manual presents a state-of-the-art CRISPR-Cas: A Laboratory Manual CRISPR Cas FM 1 - Cold Spring Harbor Laboratory Press CRISPRCas A LABORATORY MANUAL All rights reserved 2016 by Cold Spring Harbor Laboratory Press Cold Spring Harbor New York Printed in the United States of America CRISPR-Cas: A Laboratory Manual Download a Free Excerpt from CRISPR-Cas: A Laboratory Manual: Featuring contributions from leading figures in the CRISPRCas field this laboratory manual CRISPR-Cas: A Laboratory Manual - CSHL Press CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna University of California Cold Spring Harbor Laboratory Meetings and Courses DNA Learning Center
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